Characterization of membrane occupation and recognition nexus repeat containing 3, meiosis expressed gene 1 binding partner,in mouse male germ cells

Mammalian spermatogenesis is a well-organized process of cell development and differentiation. Meiosis expressed gene 1 (MEIG1) plays an essential role in the regulation of spermiogenesis. To explore potential mechanisms of MEIG1''s action, a yeast two-hybrid screen was conducted, and several potential binding partners were identified; one of them was membrane occupation and recognition nexus repeat containing 3 (MORN3). MORN3 mRNA is only abundant in mouse testis. In the testis, Morn3 mRNA is highly expressed in the spermiogenesis stage. Specific anti-MORN3 polyclonal antibody was generated against N-terminus of the full-length MORN3 protein, and MORN3 expression and localization was examined in vitro and in vivo. In transfected Chinese hamster ovary cells, the antibody specifically crossed-reacted the full-length MORN3 protein, and immunofluorescence staining revealed that MORN3 was localized throughout the cytoplasm. Among multiple mouse tissues, about 25 kDa protein, was identified only in the testis. The protein was highly expressed after day 20 of birth. Immunofluorescence staining on mixed testicular cells isolated from adult wild-type mice demonstrated that MORN3 was expressed in the acrosome in germ cells throughout spermiogenesis. The protein was also present in the manchette of elongating spermatids. The total MORN3 expression and acrosome localization were not changed in the Meig 1-deficient mice. However, its expression in manchette was dramatically reduced in the mutant mice. Our studies suggest that MORN3 is another regulator for spermatogenesis, probably together with MEIG1.     

腹腔镜保守性手术后配合药物治疗子宫内膜异位症效果观察

目的 :探讨子宫内膜异位症腹腔镜保守性手术后采用米非司酮或妈富隆的治疗效果。方法 :将我院2012年11月~2013年12月采用腹腔镜保守性手术治疗的84例子宫内膜异位症患者随机分为观察组和对照组,每组42例,对照组术后采用妈富隆治疗,观察组术后采用米非司酮治疗,比较两组的治疗效果。结果 :两组患者在疗效、药物副反应、症状及体征评分、病灶大小等方面均无统计学意义。观察组用药3个月后的雌激素E2、LH、FSH波动水平小于对照组,有统计学意义。结论 :子宫内膜异位症腹腔镜手术后采用米非司酮和妈富隆均能有效降低复发率,米非司酮对卵巢功能影响小,妈富隆不良反应少。